Magnus Sheffield and Brandon Rivera crossed the line in the top 10 following a heavy day of climbing at Settimana Coppi e Bartali.
The second stage saw an elite lead GC group form in the closing stages ahead of the fourth and final ascent of the Sogliano al Rubicone climb.
Sheffield launched early on the final ramp in a bid to overhaul lone leader Paul Double (Jayco AlUla) and would eventually cross the line three seconds behind the victorious Brit.
Rivera was also present in the group and came home a further two seconds back. The result puts both Grenadiers firmly in the GC mix, with the climbing set to continue on Thursday.
Caleb Ewan began the day in the leader’s jersey following his sprint victory heroics on the opening day. The Australian will continue to rack up important race kilometres during the remainder of the race, helping his teammates ahead of the climbing tests to come.
Egan Bernal took seventh place on stage three at the Volta a Catalunya as he and Laurens De Plus moved into the top-10 overall.
Bernal was among the riders contesting the uphill sprint at La Molina, which saw the pair of Juan Ayuso (UAE Team Emirates - XRG) and Primoz Roglic (Red Bull - Bora - Hansgrohe) push clear in the final metres. Ayuso would take a narrow victory and move into the overall race lead.
The Grenadiers remained well positioned through the day, with Geraint Thomas also present in the lead group as the final climb began to kick up.
With four stages to go, De Plus holds seventh overall with Bernal 10th, both 17 seconds back on Ayuso.